Abstract The phosphorylation of histone H2AX in Serine 139 (gamma‐H2AX) marks regions of DNA double strand breaks and contributes to the recruitment of DNA repair factors to the site of DNA damage. Gamma‐H2AX is used widely as DNA damage marker in vitro, but its use for genotoxicity assessment in vivo has not been extensively investigated.

Histone H2A.X (H2AX) is a member of the histone H2A family which is one of the four core histones making up the nucleosome core particle. In eukaryotes, DNA double strand breaks (DSBs) have been shown to trigger the phosphorylation of serine 139 at the …

H2AX is a variant of histone H2A, one of the histone core molecules forming the nucleosome, and is a vital component in repairing DNA damage (1-4). Therefore, the analysis of H2AX phosphorylated (γH2AX) may be possibly used as biomarker of genotoxicity and genomic instability with a number of applications in human epidemiology. However, the lack of an experimental standard leads to a wide heterogeneity in the results obtained and their interpretation, affecting the reliability of the assay. Target Information Histone H2A.X (H2AX) is a member of the histone H2A family which is one of the four core histones making up the nucleosome core particle. In eukaryotes, DNA double strand breaks (DSBs) have been shown to trigger the phosphorylation of serine 139 at the carboxy terminus of histone H2AX resulting in gamma-H2AX. H2AX (H2A histone family member X) helps to maintain genome stability.

Gamma h2ax

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Phospho-H2AX or γ-H2AX- is a marker of DNA double-stranded breaks and can therefore be used to monitor DNA repair after, for example, irradiation. In addition, positive staining for phospho-H2AX may indicate genomic instability and telomere dysfunction in tumour cells and tissues.

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Keywords: Gamma-H2AX, High-content screening, Image processing, Hough transform, dosimetry 1. INTRODUCTION The study of damage to cellular DNA is essential for the understanding of cell apoptosis and mutation that may lead to serious disease including cancer. Changes in the genome caused by a single double strand break (DSB) may be enough

As a result of DSBs, H2AX becomes phosphorylated at serine-139 (p Ser139). Abstract Phosphorylation of the Ser-139 residue of the histone variant H2AX, forming γH2AX, is an early cellular response to the induction of DNA double-strand breaks. Detection of this Gamma H2AX (gammaH2AX) is the phosphorylated version of histone H2AX and is a marker for double-stranded breaks (DSBs) caused by DNA damage (1-4). H2AX is a variant of histone H2A, one of the histone core molecules forming the nucleosome, and is a vital component in repairing DNA damage (1-4). Therefore, the analysis of H2AX phosphorylated (γH2AX) may be possibly used as biomarker of genotoxicity and genomic instability with a number of applications in human epidemiology. However, the lack of an experimental standard leads to a wide heterogeneity in the results obtained and their interpretation, affecting the reliability of the assay.

gamma-H2AX has already been investigated 2012-07-23 gamma-H2AX dephosphorylation by protein phosphatase 2A facilitates DNA double-strand break repair. Chowdhury D, Keogh MC, Ishii H, Peterson CL, Buratowski S, Lieberman J. Mol Cell.
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Phosphorylation of histone protein H2AX on serine 139 (gamma-H2AX) occurs at sites flanking DNA double-stranded breaks (DSBs) and can provide a measure of the number of DSBs within a cell. We gamma-H2AX Recombinant Monoclonal Antibody [BLR053F] Reactivity Human, Mouse. Applications WB, IP, IHC, ICC, IHC-IF. Immunogen surrounding Serine 140.

2019-09-27 Histone H2AX phosphorylation on a serine four residues from the carboxyl terminus (producing gammaH2AX) is a sensitive marker for DNA double-strand breaks (DSBs). DSBs may lead to cancer but, paradoxically, are also used to kill cancer cells. Using gammaH2AX detection to determine the extent of DSB induction may help to detect precancerous cells, Gamma H2AX (γH2AX) is the phosphorylated version of histone H2AX and is a marker for double-stranded breaks (DSBs) caused by DNA damage.
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2016-03-31

PMID 16310392 : Tyrosine dephosphorylation of H2AX modulates apoptosis and survival decisions. HT gamma -H2AX Pharmacodynamic Assay 4418-096-K 2 Citations Submit a Review Datasheet / COA / SDS For the quantitation of gamma-H2AX levels in peripheral blood mononuclear cells, tissue culture cells, and tissue biopsies Kinner A, Wu W, Staudt C, Iliakis G (2008) Gamma-H2AX in recognition and signaling of DNA double-strand breaks in the context of chromatin.